The smallest of the URF's (URFA6L), is a 207-nucleotide. The NH-terminal portion of the adenosinetriphosphatase subunit 6 gene has been identified as the animal equivalent of yeast H+-ATPase subunit 8 gene. Functions of the other URFs are still elusive. Immunoprecipitation reactions have proved that URFs 1 through 5 and URF4L encode subunits of a NADH dehydrogenase called complex 1, a complex that has many subunits synthesised in the cytoplasm. The reactions used NADH-ubiquitous oxidoreductase from bovine heart as well as enzyme fractionation studies.
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Thursday, September 24, 2015
The smallest of the URF's (URFA6L), is a 207-nucleotide. The NH-terminal portion of the adenosinetriphosphatase subunit 6 gene has been identified as the animal equivalent of yeast H+-ATPase subunit 8 gene. Functions of the other URFs are still elusive. Immunoprecipitation reactions have proved that URFs 1 through 5 and URF4L encode subunits of a NADH dehydrogenase called complex 1, a complex that has many subunits synthesised in the cytoplasm. The reactions used NADH-ubiquitous oxidoreductase from bovine heart as well as enzyme fractionation studies.
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Oxidoreductases consist of a large class of enzymes catalyzing the transfer of electrons from an electron donor (reductant) to an electron acceptor (oxidant) molecule, generally taking nicotinamide adenine dinucleotide phosphate (NADP) or nicotinamide adenine dinucleotide (NAD) as cofactors. oxidoreductase introduction
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